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[KO Validated] Smad3 Rabbit mAb
[KO Validated] Smad3 Rabbit mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M0571
Reactivity:Human,Mouse
Applications:WB,IHC-P,IF/ICC,IP,ELISA
Modifcation:Unmodified
Source:Rabbit
Storage and Stability:Store at -20℃/ 1 year. Avoid freeze / thaw cycles.

$150
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Recombinant Antibody
20ul Size Available
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[KO Validated] Smad3 Rabbit mAb

Immunohistochemistry analysis of paraffin-embedded Human liver using [KO Validated] Smad3 Rabbit mAb (A23491) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

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Product Details
Product Name
[KO Validated] Smad3 Rabbit mAb
Description
The SMAD family of proteins are a group of intracellular signal transducer proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. The SMAD3 protein functions in the transforming growth factor-beta signaling pathway, and transmits signals from the cell surface to the nucleus, regulating gene activity and cell proliferation. This protein forms a complex with other SMAD proteins and binds DNA, functioning both as a transcription factor and tumor suppressor. Mutations in this gene are associated with aneurysms-osteoarthritis syndrome and Loeys-Dietz Syndrome 3.
Catalog No
M0571
Reactivity
Human, Mouse
Applications
WB, IHC-P, IF/ICC, IP, ELISA
Application Dilutions
WB,1:500 - 1:1000 | IHC-P,1:50 - 1:200 | IF/ICC,1:50 - 1:200 | IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells | ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Modifcation
Unmodified
Source
Rabbit
Isotype
IgG
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 119-219 of human Smad3 (NP_005893.1).
Calculated MW (kDa)
48kDa
Observed MW (kDa)Gene Name
52kDaSMAD3
Other Name
LDS3; mad3; LDS1C; MADH3; JV15-2; hMAD-3; hSMAD3; HSPC193; HsT17436; d3
Gene ID
4088
Human Swiss Prot
P84022
Formulation
PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Storage and Stability
Store at -20℃/ 1 year. Avoid freeze / thaw cycles.
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Primary Antibodies

    • ELISA Kits

    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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